Is it not reasonable because he is arguing with Rishonim/Acharonim?
Yes, but it’s more than that. He says tzorich iyun on the Rema; it’s one thing to say a pshat not in accordance with the rishonim, it’s quite another to ask a kashya on the Rema for not doing so.
Also, if he means to argue on the Kol Bo, how can he ask on the Rema based on the Kol Bo?
Furthermore, R’ Chaim never said a sevara to argue on guf naki; why would there be a tzorich iyun on the Kol Bo?
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Since the B”Y/K”B are clearly going with guf naki, and the Rema, even according to R’ Chaim, is going with their pshat in the sugya, how is it shayach to ask on him from the Targum Yonoson?
As an aside, if he’s saying a pshat independent of the standard mehalach in the sugya, why mention the Gr”a?